Another way of thinking about Blast2go

This is a created article in which the information may have evolved or changed.

Blast2go is the use of sequence similarity to get go information, and many of the online software is either for a protein, or only online operation, there is only windows, annoying-

But if the name corresponds to what happens, try a record as follows:

Our sequence first carried out the Blast NR Library is a comparison of the protein sequence, the name of the GI number and refseq number;

NCBI FTP Inside there is a gene2go file, found that is geneid corresponding to the go number;

And from the FTP inside found Gene2refseq file, is geneid corresponding GI number;

OK, so we can put these three in series, the results found more than 350 correspondence, and Blast2go results have 303,\ (^o^)/~!

But after I took the two to the intersection, I found only 5! There are only 5 of them! Only 5!!! Instant Felling happy ...

So I was wondering if there are several things to consider:

First of all, the filter, we blast parameters are set, of course, can filter part, the program is in the database and some of the best, but Blast2go certainly did another filter;

The second is the database, Blast2go may take not only NCBI inside the gene2go, there are other, still do not understand;

Finally, is the program, is not blast2go with the level of association to exclude part, dug up a part, I was drunk!!!

There is 2016 years, do a small summary, although a few months did not update (upset things), but finally temporarily resolved, next year will be what?