Transferred from: https://zhuanlan.zhihu.com/p/20702684
1. Basic Concepts
- Flowcell refers to the position of the sequencing reaction when the Illumina is sequenced, 1 Flowcell contain 8 lane
- Lane has 8 lanes on each Flowcell, for sequencing reactions, reagents to be added, elution, etc.
- Tile Minimum unit for each sequencing fluorescence scan
- reads refers to the results of sequencing, 1 sequences are generally called 1 reads
- BP base pair base pair for measuring sequence length
- two-terminal sequencing only one sequence may be longer as 500bp, we can test each end of 150bp
- Junction said the two-terminal sequencing, the middle will be left with 200bp can not be measured, we call junction
- Adapter is a specific sequence required for sequencing, and has a function similar to primer
- Primer Primers in PCR
CDNA: Complementary DNA complementary DNA. Copies of eukaryotic organisms (in the form of mRNA) are copied to the prokaryotic cells using a reverse transcriptase, a copy of the mRNA template.
It seems to be understood, summed up tomorrow, and look at the PDF!!!
Health Letter Learning-Two generation sequencing knowledge column summary [GO]
