The revolution is not yet successful, and comrades still need to work hard

the boss did not answer questions last week. I listened to my opening report after the meeting yesterday, basically, I didn't give too many comments and suggestions on the design of the project, but I urged you to issue the Article quickly.

1. 4 * and 5 * the risks do not seem to have many results, which make people feel suspended, and the Senior Brother suggests releasing them, mainly page the result is uncertain, PCR the conditions seem unstable, and the sequencing mixing is hard to say. I should try to optimize the conditions myself.

2.Full-length single-clone cells are the most annoying and cannot be identified!WesternIt is something that gets the result quickly. Otherwise, the Cell will not always be able to identify it, so it is not a way to maintain it;

3. Cooperating with sun Lijun Cyclic Family RT-PCR Hope to have a pleasant cooperation, RT-PCR I will do it later Western To obtain indicators;

4.As instructed by the Director, the constructed vector should be used for the promotion of monoclonal antibodies. The expression of the vector should be completed as soon as possible. I have just read the data in the morning and it should be similar to that of the eukaryotic expression, however, we haven't done anything for a long time to clone the molecules. Now we have to re-Prepare the bottles and the like, let alone the competent cells. But if it works well, it will be very fast;

5. The remaining missing mutant should 5 * One set of cells, which is another month to extract the plasmid and screen. This is mainly based on the preliminary expression of the group, add some indicators with my 4 * , 5 * The total length is determined by comparison. Apoptosis Get the indicators and post articles, of course, more work will be done later.

6. in this case, I have a lot of things now, cloned, cultured, and transferred, Western , RT-PCR I should make good use of and arrange the time, let yourself enrich yourself and make full use of time. You don't have to find anything else.