Transcription group entry (5): Sequence Comparison and transcription entryTask List
Comparison Software
Hisat2 usage
Download the index file
Comparison, sorting, and Indexing
Quality Control
Load IGV, several genes
Hisat2 is compared to the genome, so gapped or splices er is used. This process has been updated. TopHat was first published seven years ago. The comparison speed of STAR is 50 times that
Entry to transcription groups (1): software installation and entry to transcriptionHISAT
Wget ftp://ftp.ccb.jhu.edu/pub/infphilo/hisat2/downloads/hisat2-2.1.0-source.zipUnzip hisat2-2.1.0-source.zipCd hisat2-2.1.0Make # install g ++ and gcc before using sudo apt installRm-f *. h *. cppSamtools
Wget https://github.com/samtools/samtools/releases/download/1.5/samtools-1.5.tar.bz2Tar xvfj samtools-1.5.tar.bz2Cd samtools-1.5./Configure # install the syst
Entry to transcription groups (2): reading articles to obtain Sequencing data, transcription sequencingHttps://trace.ncbi.nlm.nih.gov/Traces/study? Acc = PRJNA323422
Lne is a perl parameter. The result is that after perl receives your keyboard input (using the following code), It outputs a line feed and waits for the next input to be received.
Perl-lne '$ id = substr ($ _,); print "axel plugin" 'srr_acc
What is a transcription factor?
Transcription factors
What are the transcription factors that are basically introduced in transcription factors?A transcription factor (TRANSCRIPTIONFACTOR,TF) is a protein that can be combined with a specific DNA sequence to for
F5 session persistence, f5 session
In recent projects, F5 needs to be used for load balancing and relevant information should be recorded.
The following is a description of the relevant parameters in the F5 change application. Let's take a look at them. Let's pick several important parameters to learn.
Enter in the URL address bar: Such a refresh, you can see in the Firebug, only a few requests will be sent out, and almost no picture of the request, this is because the request will first check whether the local cache of the requested picture, If there is a cache and there is no expiration (the expiration can be viewed through the header of the picture request), he will not issue the picture requests.F5:f5 refresh is slightly slower than enter, for w
like you find a box of milk that has expired, so ask others, can not drink, if others say yes, you drink it, if others say no, then you have to find another box of fresh milk.As for F5 Refresh, its HTTP request message header is as follows:Host 192.168.3.174:8080User-agent mozilla/5.0 (Windows NT 5.1; rv:5.0) gecko/20100101 firefox/5.0Accept text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8Accept-language zh-cn,zh;q=0.5Accept-encoding g
, the system returns 304 not modified. After the browser receives the modification, it reads the content from the cache. If it is modified, the system returns 200.OK, and new content is returned. In this situation, you find a box of expired milk, and ask someone else if they can drink it. If someone else says yes, you will drink it. If someone else says no, then you haveFind a box of fresh milk.
For F5 refresh, the HTTP request message header is as
Pressing CTRL + F5 in Internet Explorer is a convenient way to force the page to be reloaded. All components of the page (images, CSS, JavaScript, and so on) are forcibly reloaded. it is equivalent to using an empty cache IE to access a page.
F5 is a simple refresh of the current page, click Ctrl + R, or right-click to refresh, it is the same, they have"If-modified"String. If you view
F5 (debugging) and server controls, f5 debugging server controls
I. debugging
Background:
When debugging today, I found that the website I entered is http: // ×××. com: 7813/webaspx/System/Login. aspx (because the code is in the company, I don't have it. After waiting for half a day, the page cannot be loaded ). I have encountered a problem similar to this kind of problem since I just entered a new company
milk. As for F5 Refresh, its HTTP request message header is as follows: host 192.168.3.174:8080 user-agent Mozilla /5.0 (Windows NT 5.1; rv:5.0) gecko/20100101 firefox/5.0 accept text/html,application/xhtml+xml, application/xml;q=0.9,*/*;q=0.8 accept-language zh-cn,zh;q=0.5 Accept-Encoding gzip, deflate accept-charset gb2312,utf-8;q=0.7,*;q=0.7 connection keep-alive if-modified-since Mon, 10:12:40 GMT cache-control Max-age=0 Another l
like you find a box of milk that has expired, so ask others, can not drink, if others say yes, you drink it, if others say no, then you have to find another box of fresh milk.As for F5 Refresh, its HTTP request message header is as follows:Host 192.168.3.174:8080User-agent mozilla/5.0 (Windows NT 5.1; rv:5.0) gecko/20100101 firefox/5.0Accept text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8Accept-language zh-cn,zh;q=0.5Accept-encoding g
NMF (non-negative matrix decomposition), because its decomposition of the matrix is non-negative, in some practical problems have a very good explanation, so the use is very wide. Here, I would like to introduce the application of NMF in the multi-part music. To translate the paper is the use of NMF transcription multi-part music of the mountain, simple and easy to understand how to use the NMF to the piano score translation, it is worth a look.Summar
3162 transcription ProblemsTime limit: 1 sSpace limit: 128000 KBTitle Level: Diamonds DiamondExercisesView Run ResultsTitle Description DescriptionNow to the M-Order of the book to the K-person copy (transcription), each person's transcription speed is the same, a book is not allowed to two (or more) people to copy, to each person's book, must be continuous, such
TCP/IP network programming (transcription Note 5) –select and IO multiplexingUsing fork () to generate a child process can reach a server-side request that can respond to multiple clients at the same time, but this has drawbacks:Requires a lot of computing and memory space, each process must have a separate memory space, data exchange is also cumbersome (IPC, such as pipelines)IO Multiplexing:Ethernet bus structure is also used multiplexing technology
Cyclic redundancy check, transcription
Cyclic redundancy check, transcriptionAdd an FCS (frame checksequence, frame check sequence) to each data block (called frame) for the transmit/receive unit to compare frames correctly or notCyclic redundancy check: CRC (cycli redundancy check)
M represents the transmitted data
K indicates the length of the transmitted data
N-bit redundancy code, can be obtained with M * 2^n
The number (N+K) i
TCP/IP network programming (transcription note 3) – Zombie process and multitasking concurrent server table of Contents
The production of zombie processes
Avoid zombie processes
Signal
Multi-tasking Concurrent server
The production of zombie processes
Child process exits first, parent process does not exit ==> zombie process
The parent process exits first, the child process does not exit the ==> child process is
TCP/IP network programming (transcription note 3) – Zombie process and multitasking concurrent server table of Contents
The production of zombie processes
Avoid zombie processes
Signal
Multi-tasking Concurrent server
The production of zombie processes
Child process exits first, parent process does not exit ==> zombie process
The parent process exits first, the child process does not exit the ==> child process is
TCP/IP network programming (Transcription Note 2) –udptable of Contents
Server
Client
Connect
Source: "TCP/IP network Programming"Transcription
TCP protocol to provide services to 10 clients, 10 server-side sockets are required in addition to listen sockets(accept), but in UDP, both the server side and the client require only 1 socketsUDP client does not need bind, when calling t
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