jpg can‘t be recognized by pyautogui, but png can

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Python 3.4.3 (v3.4.3:9b73f1c3e601, Feb 24 2015, 22:43:06) [MSC v.1600 32 bit (Intel)] on win32
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>> New_locat = pyautogui.locateOnScreen(‘C:/Python34/PDF2JPG/CLICK_NEW.jpg‘)
Traceback (most recent call last):
File "<pyshell#0>", line 1, in <module>
New_locat = pyautogui.locateOnScreen(‘C:/Python34/PDF2JPG/CLICK_NEW.jpg‘)
NameError: name ‘pyautogui‘ is not defined
>> import pyautogui
>> New_locat = pyautogui.locateOnScreen(‘C:/Python34/PDF2JPG/CLICK_NEW.jpg‘)
>> New_locat
>> button7x, button7y = pyautogui.center(New_locat)
Traceback (most recent call last):
File "<pyshell#4>", line 1, in <module>
button7x, button7y = pyautogui.center(New_locat)
File "C:\Python34\lib\site-packages\pyscreeze__init__.py", line 407, in center
return (coords[0] + int(coords[2] / 2), coords[1] + int(coords[3] / 2))
TypeError: ‘NoneType‘ object is not subscriptable
>> New_locat = pyautogui.locateOnScreen(‘C:/Python34/PDF2JPG/CLICK_NEW.png‘)
>> New_locat
(1027, 389, 70, 35)
>> button7x, button7y = pyautogui.center(New_locat)
>> button7x
1062
>> button7y
406
>>

jpg can‘t be recognized by pyautogui, but png can