Ion torrent, Proton

Https://www.youtube.com/watch?v=6Is3W7JkFp8

NGS's video is pretty good. A professor of cancer is talking about

Ion torrent™next-generation Sequencing

Next-generation sequencing (NGS) utilizes massively parallel sequencing to generate thousands of megabases of sequence inf Ormation per day, opening doors to new-studies that were once difficult-accomplish in a practical manner. Powered by semiconductor chips, Ion torrent™sequencing technology are simpler, faster, and more cost-effective and scalabl E than other benchtop next-generation sequencing technologies. Experience the speed, scalability, and simplicity of Ion torrent™sequencing.

Ion Proton™system for next-generation sequencing

Comparison of sequencing techniques:

https://www.ebi.ac.uk/training/online/course/ebi-next-generation-sequencing-practical-course/ What-next-generation-dna-sequencing/improveme

Unlike Illumina and 454, ion torrent and ion proton sequencing do don't make use of optical signals. Instead, they exploit the fact that addition of a dntp to a DNA polymer releases an H + ion.

As in other kinds of NGS, the input DNA or RNA was fragmented, this time ~200bp. Adaptors was added and one molecule is PLA CED onto a bead. The molecules is amplified on the bead by emulsion PCR. Each bead is placed to a single well of a slide.

Like 454, the slide are flooded with a single species of dntp, along with buffers and polymerase, one NTP at a time. The ph is detected are each of the wells, as each H + ion released would decrease the ph. The changes in PH allow us to determine if that base, and how many thereof, is added to the sequence read.

Simply put, ion Proton recognizes that the base is through the ph value, not the optical signal.

Freemao

Fafu

Ion torrent, Proton