How to calculate the sequencing depth

If the two-terminal sequencing gets these two files:

L2y-0_tgacca_l002_r1.fastq.gz

L2y-0_tgacca_l002_r2.fastq.gz

If you use Wc-l to see the number of rows in the GZ file, the returned result is incorrect

General use FASTQC to see the quality how:

/SHARE/HISEQ/SAMPLEPROCESS/FASTQC/FASTQC 20_gtggcc_l000_r1.fastq.gz

You just need to put one back, and the auto two will run out.

Then look at the results, with the total number of series:

This one is single-ended.

Then the depth of coverage is:

(Total_sequences * 2) * Length of sequencing/size of genome

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How to calculate the sequencing depth