Steroid Receptor co-activating-1 (SRC-1) is a group

Steroid Receptor coactivator-1 is a histone acetyltransferase

• Thomas E. Spencer
• , Guido jenster
• , Mark M. burcin
• , C. David allis
• , Jianxin Zhou
• , Craig A. mizzen
• , Neil J. McKenna
• , Sergio A. onate
• , Sophia Y. Tsai
• , Ming-Jer Tsai
• & Bert W. O 'Malley

Nature Volume 389, Pages 194-198 (11 September 1997)

 

 

Steroid Receptor co-activating-1 (SRC-1) is a group

 

Main terms:

300/ CBP-associated factor(Pcaf), Also knownK (lysine) acetyltransferase 2B(Kat2b), Is a human gene and Transcriptional coactivator associated with p53.

White and 300are large nuclear proteins that bind to sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. the protein encoded by the pcaf gene associates with 300/ CBP. it has in vitro and in vivo binding activity with white and 300s, and competes with E1A for binding sites in 300/ white S. it has histone acetyl transferase activity with core histones and nucleus OSome core particles, indicating that this protein plays a direct role in Transcriptional Regulation.^[6]

Coactivator:ACoactivatorIs a type of transcriptional coregulator that binds to an activator (A trancoder factor) to increase the rate of trancoder of a gene or set of genes. the activator contains a DNA binding domain that binds either to a DNA Promoter site or a specific DNA Regulatory Sequence called an enhancer. binding of the activator-coactivator complex increases the speed of trancoder by recruiting General transhummachinery to the promoter, therefore increasing gene expression. the use of activators and coactivators allows for highly specific expression of certain genes depending on cell type and developmental stage.

Some coactivators also have histone acetyltransferase (HAT) activity. hats form large multiprotein complexes that weaken the association of histones to DNA By acetylating the N-terminal histone tail. this provides more space for the transhummachinery to bind to the promoter, therefore increasing gene expression.

Activators are found in all living organisms, but coactivator proteins are typically only found in eukaryotes because they are more complex and require a more intricate mechanic for gene regulation. in eukaryotes, coactivators are usually proteins that are localized in the nucleus.

Histone acetyltransferases(Hats): Are enzymes that acetylate conserved lysine amino acids on histone proteins by transferring an acetyl group from acetyl-CoA to form ε-N-Acetyllysine. DNA is wrapped around und histones, and, by transferring an acetyl group to the histones, genes can be turned on and off. In general, Histone Acetylation increases gene expression.

Family	Organism	Associated Complexes	Substrate Specificity	Structural Features

Pcaf

H. sapiens

Pcaf

H3, H4

Bromodomain

P300

H. sapiens

H2A, H2B, H3, H4

Bromodomain

CBP

H. sapiens

H2A, H2B, H3, H4

Bromodomain

SRC-1

H. sapiens

Actr/SRC-1

H3, H4

Tafii250 (taf1)

S. cerevisiae-H. sapiens

Tfiid

H3, H4, (H2A)

Bromodomain

Before using the API, run the following command:

The Win32/CBP hats have larger hat domains (about 500 residues) than those present in the gnat and myst families.^{[5] They also contain a bromodomain as well as three cysteine/histidine-rich domains that are thought to mediate interactions with other proteins. the structure of 300s/White s is characterized by an elongated globular domain, which contains a seven-stranded β-sheet in the center that is surrounded by nine α-helices and several loops.[7] The structure of the central core region associated with acetyl-CoA binding is conserved with respect to gnat and myst hats, but there are too structural differences in the regions flanking this central core. overall, the structural data is consistent with the fact that p53/CBP hats are more promiscuous than gnat and myst hats with respect to Substrate Binding.}

CREB-binding protein (CBP ):

　　CREB-binding protein, also known as crebbp or CBP, is a protein that in humans is encoded byCrebbpGene.^{[5][6] The CREB protein carries out its function by activating trancoder, where interaction with trantrans factors is managed by one or more CREB domains: the Nuclear Receptor interaction domain (RID ), the Kix domain (CREB and myb interaction domain), the cysteine/histidine regions (taz1/logs and taz2/CH3) and the interferon response binding domain (ibid ). the CREB protein domains, Kix, taz1 and taz2, each bind tightly to a sequence spanning both transactivation domains 9 aatads of transtmfactor p53.}

Ep300 (p300 ):

Histone Acetyltransferase p300also known as p300hat or E1A-associated protein p300( where E1A = adenovirus early region 1a) also known as ep300 or p300is an enzyme that, in humans, is encoded byEp300Gene.^{[5] It functions as histone acetyltransferase that regulates tranates of genes via chromatin remodeling this enzyme plays an essential role in regulating cell growth and division, prompting cells to mature and assume specialized functions (differentiate), and preventing the growth of cancerous tumors. the 300protein appears to be critical for normal development before and after birth.}

Cos: Are maid-like cell lines derived from monkey kidney tissue. COS cells are obtained by immortalizing CV-1 Cells^{[1] with a version of the SV40 virus that can produce large t antigen but has a defect in Genomic Replication.[2] The CV-1 cell line in turn was derived from the kidney of the African green monkey.[3]}

Cos cell lines is a cell line derived from the kidney fiber cells of the green monkey and transformed by the SV40 virus gene. the most common cos cell lines are cos-1 and cos-7. they are generally used in virus and Gene Transfer Studies and cell gene transcription studies.

 

Gal4 transtmfactorIs a positive regulator of gene expressionGalactose-induced genes.^[1]

The gal4 protein represents a large fungal family of transtmfactors,Gal4 family, Which between des over 50 members in the yeastSaccharomyces cerevisiaeE.g. oaf1, pip2, pdr1, pdr3, il-3.

Gal4 Domains

DNA binding domain: localised to the N-terminus, belongs to the Zn (2)-C6 fungal family, which forms a Zn-cysteines thiolate cluster. the gal4 DNA binding domain recognized specifically response element in gal1 promoter.

Gal4 activation domain: localised to the C-terminus, belongs to the nine amino acids transactivation domain family, 9 aatad, together with oaf1, pip2, pdr1, pdr3, but also p53, E2A, MLL.

Transactivation: Activation factors (such as transcription and translation activation factors) regulate gene expression, which may be activated or enhanced due to direct or indirect binding of proteins.

 

Abstract (this article was published in 1997)

Steroid Receptor and co-activating proteins are considered to stimulate Gene Expression by promoting the stable starting structure of the transcription factor group.

However, it is still unclear how these transcription factors break through the transcription inhibition of the chromosome and regulate the transactivation of the special gene network in the body.

There is evidence that in vivo there is a binding relationship between acetyl and transcription, and the specific HMAC targets DNA-bound HMAC to overcome the inhibitory effect of chromosome on gene expression.

SRC-1 is a co-activator of some hormones receptor hyperfamilies.

The results show that SRC-1 has acetyl transferactivity and interacts with another hat and pcaf.

The hat activity of SRC-1 in C end and specific to H3 and H4.

SRC-1 and pcaf are bound to the DNA binding group of specific sites due to the binding of the steroid receptor, it is the mechanism by which the steroid receptor binding compound plays the activation function and associated co-activating agents enhance stable initial Compound Formation, so it increases the transcription of the exclusive gene in the transcription inhibition of the chromosome template.

 

Steroid Receptor co-activating-1 (SRC-1) is a group