The answer is yes.
1. Install patch packages on all servers presentProgramUntil you encounter the following information:
You must run Setup to install new binary files for every server in your server farm. if you have multiple servers in your server farm, run setup and the Configuration Wizard on the other servers now, and then return to this server and click OK to continue.
2. you need to first run Configuration Wizard on the server that stores the Management Center site. in this way, you have completed the task of upgrading the database. when you run Configuration Wizard on another server, WFE or application server will connect to the database that has been upgraded, instead of trying to upgrade the database.
3. Execute Configuration Wizard on other Web Front-ends or application servers to complete the upgrade process.
The details of the upgrade are described in the planning and deploying Service Pack 1 for Microsoft Office Sharepoint Server 2007 in a multi-server environment document.
Including installing the WSS upgrade package, installing the moss upgrade package, how to back up and so on.
Source:
Planning & deploying Service Pack 1 for Microsoft Office Sharepoint Server 2007 in a multi-server environment
Http://technet.microsoft.com/en-us/library/cc262996.aspx