Anti-keratin Antibody (AKA), 1979 Young and other found in RA serum has an antibody that can react with the rat esophagus stratum corneum, and the RA is specific, named AKA. Directory
- 1 overview
- 2 Detection method
- 3 Clinical Significance
Overview Edit Anti-keratin Antibody (AKA), 1979 Young and other found in RA serum has an antibody that can react with the rat esophagus stratum corneum, and the RA is specific, named AKA. 1989 Vincent, etc., it is more appropriate to rename aka to Anti-cuticle antibody. AKA can occur several years before the onset of RA, so there is an early diagnostic value.detection method
Aka Detection method: 6 weeks old male Wistar rat esophagus in the middle of the lower 1/3 as an antigen, do frozen section, thick 4μm~5μm,-70℃ preservation reserve. Add 1:20 diluted serum, moist box 37 ℃ incubate for 30 minutes, PBS rinse, blow dry, add 1:20 diluted fluorescein labeled sheep Anti-human igg,37℃ incubate for 30 minutes, rinse, blow dry, buffer glycerin seal, observe under fluorescence microscope. Criterion: The linear or lamellar fluorescence of the typical rule of stratum corneum is positive.Clinical significance
(1) RA patients: APF positive rate is 41.3%, specificity is 97.8%. (2) Normal person: Healthy adult is 2%. (3) Other rheumatism: Positive rate 2.2%. It is worth noting that:APF, aka, and anti-sa antibodies may appear before Ra is diagnosed. The antibody positive group was diagnosed as RA after half and more than half of the follow-up, and the few diagnosed as non-Ra。 Conversely, the antibody negative group was diagnosed as RA, the number of cases diagnosed as non-RA increased significantly. The anti-RA33/36 antibody is roughly the same as three autoantibodies, but it is less significant than the early diagnosis of the three antibodies. Therefore, it is concluded that these autoantibodies may appear in the early stages of RA, and that the positive autoantibodies in patients with undiagnosed joint pain/arthritis are helpful for early diagnosis of RA. APF, AKA, anti-sa antibodies and anti-ra33/36 antibodies can be found in patients with undiagnosed joint pain/arthritis (including those not yet diagnosed with RA, but after follow-up of RA patients) and have some value in early diagnosis of RA. With 1 antibodies positive to determine whether the patient will be diagnosed in the future of RA risk, and more than 2 antibodies at the same time more grasp, more than 3 antibodies appear at the same time, almost can be said that 100% will be developed into RA. That is, the more positive number of autoantibodies, the more likely to diagnose RA after follow-up. Especially for RF negative RA patients with supplementary diagnostic significance.
Anti-keratin antibody anti-keratin Antibodies
