Anti-methionine peptide antibody

Anti-methionine peptide antibody (CCP) is a cyclic poly-protein peptide fragment, is a type of IgG-based antibody, rheumatoid arthritis (RA) has a good sensitivity and specificity, and anti-CCP-positive RA patients with bone damage than anti-CCP antibody negative people serious. Anti-methionine peptide antibody is a kind of rheumatism autoantibodies system, rheumatoid arthritis (RA) has a highly specific citrulline-related autoantibodies system including aka, APF, AFA, anti-CCP antibody, these antibodies to early RA diagnosis has a better specificity. The higher these indicators, the greater the likelihood of rheumatic disease.

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Universal Name: anti-methionine peptide antibody

English Name: Resistance to ring citrulline peptide antibodies

English Name: CCP

Catalogue

• 1 overview
• 2 Biological characteristics
• 3 History of research
• 4 Detection method
• 5 Clinical significance

  Anti-methionine peptide antibody-overview

Anti-methionine peptide antibody (CCP) is a cyclic poly-protein peptide fragment, is a type of IgG-based antibody, rheumatoid arthritis (RA) has a good sensitivity and specificity, and anti-CCP-positive RA patients with bone damage than anti-CCP antibody negative people serious.

Rheumatoid arthritis (rheumatoid arthritis,ra) is a common chronic autoimmune disease. Around 1% of people around the world suffer from this disease. RA can cause pain, stiffness and swelling in the joints. In the 2 years of onset, irreversible bone and joint injury may occur, and can cause a variety of complications, such as neurological diseases, pericarditis, lymphadenitis and so on. Therefore, early diagnosis of RA and the treatment of medication to relieve the disease is very important to control the condition. 1998, foreign, the first reported anti-peptide antibody (anti-cyclic cirullinated peptide antibodies, anti-CCP antibody) detection, RA has a high sensitivity and specificity, is a new serum marker RA. Anti-cyclic citrulline antibody-biological characteristics

1AtlasPeptides synthesized from the cDNA sequence of Filaggrin in 1998, such as Schellekens and Girbal Neuhause, confirm that citrulline residues are essential components of rheumatoid arthritis-specific anti-intermediate silk-related protein (Filaggrin) antibody recognition table bits. By analyzing the Filggrin amino acid sequence of each serial number in the gene library, a peptide chain with citrulline instead of 20 amino acid residues of arginine was synthesized. Some tests showed that Citrulline was the main constituent antigen determinant of anti-filaggrin antibody in serum of RA patients. In 2000, Schellekens replaced a two serine peptide chain of 19 amino acid residues with cysteine, forming a disulfide bond with a similar structure to beta-rotation, and synthesizing the cyclic citrulline peptide (cyclic citrullinated PEPTIDE,CCP). The sensitivity and specificity of anti-peptide antibody in serum of RA patients were detected by Elisa method using cyclic citrulline peptide as antigen matrix.

Anti-methionine peptide antibody-a brief history of research

1AtlasAnti-citrulline peptide antibody (CCP) Elisa method was established in 1998, such as Schellkens to assist the diagnosis of RA detection method, the technology appeared, immediately received attention, there is now a supply of goods. According to the cDNA sequence of anterior filament protein, the 9~19 amino acid short peptide which may have antigen epitope was synthesized and modified by guanidine-ammonia acidification, and the Antigen fragment was screened by Elisa.

Anti-methionine peptide antibody-detection method

The anti-CCP antibody in serum of patients can be detected qualitatively or quantitatively by Elisa. Jansen Al et al. the determination of anti-CCP antibody in serum of patients with different types of multi-joint disease predicts that anti-CCP antibodies greater than 50au/ml can have higher sensitivity and specificity to early rheumatoid arthritis. Bizzaro N detected the level of anti-CCP antibody in RA patients, and the results also showed that the critical value of anti-CCP antibody was 50au/ml. And the average level of anti-CCP antibody in serum of RA patients was 1100au/ml* (range: 57~3419au/ml. The average level of anti-CCP antibody in control group was only 6.8AU/ML (range: 1~39au/ml). The diagnostic value of anti-CCP antibody to RA patients was further confirmed.

The Elisa method has a commodity reagent box. (e.g.: German Aeskulisa anti-peptide antibody RA/CP ELISA Assay Kit: Kit size 96 person/box suitable specimen: Suspected rheumatoid arthritis patient serum suitable for large-scale specimen screening, can be automated detection, results objective, data archiving. ) Anti-methionine peptide antibody-clinical significance

Clinical data show that the CCP antibody has a very high specificity and sensitivity to RA (68%~75%), even in early RA patients, the sensitivity of 40%~60%. Some scholars also believe that CCP antibody is not only an early diagnostic index of RA, but also a sensitive index to identify erosive and non-erosive RA, and CCP antibody-positive people usually appear or develop into more severe joint bone damage than those with negative antibody. However, several recent studies have shown that the sensitivity of CCP antibodies to RA in 40%~50%, less than RF, combined with the detection of RF and CCP antibodies, will significantly improve the sensitivity of diagnosis.
Although the pathogenesis of anti-CCP antibodies in RA has not yet been clarified, the early diagnosis and progression markers as a disease show a better prospect. Anti-CCP antibodies are mainly IgG antibodies, the specificity of Ra is about 96%, positive in the early stage of RA, and has a high positive pre-reported. Mediwake R et Anti-ra33 and anti-CCP antibodies were measured in SLE and RA patients who had joint injuries, and the results showed that anti-CCP antibodies were only high in RA and could be differentiated from patients with traumatic arthritis. In addition, some reports suggest that anti-CCP antibodies, as sensitive markers of RA, have the ability to differentiate between traumatic and non-traumatic joint diseases. But Bas s and so forth different points of view, through their clinical trials, that anti-CCP antibodies are specific indicators of RA early diagnosis, and RF can be used as a better marker of disease damage severity. Therefore, joint detection of anti-CCP antibody and RF is of great significance to the diagnosis and prognosis of RA. Anti-CCP antibody in addition to early diagnosis of RA, the prognosis of the disease evaluation is also important. In patients with diagnosed RA, the more negative anti-CCP antibody was more likely to develop multiple joint injuries. After 1-6 years ' follow-up of hundreds of patients with RA who had 1 years of onset of disease, kroot Ejja, through continuous observation on the scores of diseases activity, health assessment questionnaires and imaging scores, the anti-CCP-positive RA patients with negative bone damage were serious.

Anti-methionine peptide antibody