from: https://secure.clcbio.com/helpspot/index.php?pg=kb.page&id=113
Can I Run a local BLAST search again multiple BLAST databases simultaneously? (Blast, BLASTN, BLASTP, BLASTX, serveral databases)
By default, you'll be offered single BLAST databases to search via the CLC Workbench BLAST interface.
By creating a simple text file outside the Workbench, and saving it the appropriate location, you can search multiple data Bases in a single search. To does this:
1) Create a text file and name it with the suffix . nal If your databases were created from nucleotide sequences, or the suffix . pal If your databases were created from peptide sequences.
Attached is a file showing the type of the format this file should take. Just Replace the names of the databases in that file (which is in quotes), with the names of databases for use. You can add as many databases to that list as you like, just as long as you keep the same type of formatting as in the Exa Mple file.
2) Put This file to the same folder on your system as your databases is stored in.
Please note that steps 1 and 2 is done outside the Workbench.
Now if you go to run a search using your Workbench, one of the databases offered to your for BLASTN, blastx or Tblastx s Hould is the same name of the nal file you created (but without the. nal suffix). If you run a BLASTP or TBLASTX search and then one of the databases offered to you should has the same name as the. pal file You created (but without the. pal suffix).
Example.nal
#
# Alias file created 11/21/2011 16:25:58
#
TITLE My Bunch of databases
dblist "Bigstaticdatabase" "Smalldynamicdatabase"
Can I Run a local BLAST search again multiple BLAST databases simultaneously?