Ebola virus Lab-broken

Original: Who . Laboratory Guidance for the diagnosis of Ebola Virus Diseaseinterim recommendations. [2014-9-19]. http://apps. Who INT/IRIS/BITSTREAM/10665/134009/1/who _ EVD _ GUIDANCE _lab_14. 1_eng. pdf

Important considerations for laboratory staff:

(1). Ensure that adequate stocks of suitable personal protective equipment (PPE) and United Nations specimens are available in the triple packaging system;
(2). Staff should be properly trained to wear (wear) and disassemble personal protective equipment;

(3). In accordance with national/International guidelines, participation in specimen collection staff should collect, store, package and transport diagnostic specimens.

1. Specimen collection

EVD Incubation period ( from Infection virus of the Symptom Seizures time) is 2-21 days. when the patient Start symptoms occur patients when have contagious , that Fever (temperature >38.5℃), diarrhea, signs of Bleeding ( hemorrhage). The dead man 's body is also contagious.

All cases of Ebola (probable and suspected) should be submitted to the designated treatment Ebola Centre (ETC) or appropriate health care facilities, and trained medical personnel should safely collect appropriate specimens.

Time of specimen collection:
When the patient shows symptoms that fit the specimen for the EVD molecule to be tested preferably should be taken.

If the distance from the specimen to the collection interval is less than 3 days, and the test result of the first sample is negative, the additional specimen will be required, and the second sample should be collected at least after 48 hours from the first sample.
Whole blood serological tests can be performed after 8 days of onset, and strict infection prevention and control measures, including waste treatment and disinfection symptoms, are collected throughout the process. Please refer to the WHO Laboratory Biosafety Handbook version 3 for appropriate biosafety practices.

It is recommended that the following specimens be collected for diagnosis of EVD:

In the EDTA assay, all the blood collected from the patient (minimum volume of 4 ml) is collected in the plastic tube;
In the case of blood collection is not possible, such as children, death, at this time the collection of oral swabs stored in a common transport medium. It is not recommended to use swab collection for live patients, not because of the low sensitivity of reverse transcription polymerase chain reaction (RT PCR) and antigen detection.

Samples can be stored at room temperature for up to 24 hours. If the samples can only be transported and tested within a week, they are stored in between 0-5 ℃ for further RT-PCR testing. If you are at the WHO Collaborating Centre, you can store the dry ice on the 70 ℃ for long periods. Avoid freezing blood circulation.

2. Laboratory Bio-Safety recommendations

Institutions/laboratories should conduct risk assessments and have the responsibility to mitigate biological risks. Any RNA or antibody testing of Ebola virus should be carried out in suitably equipped laboratories by staff trained in relevant technical and safety procedures. The National guidelines for laboratory biosafety should be followed in any case.
If a country does not have the appropriate biosafety capacity to conduct laboratory-confirmed cases, the specimens (suspected and probable) will be investigated and the samples sent to the designated WHO Collaborating Centre for Viral haemorrhagic fever (who cc VHF) (step 4).

Filamentous viruses are highly contagious, and they need to be closely guarded in the diagnosis of pathogens and treated specimens.

In accordance with the issues identified in the risk assessment of each process, preventive measures and engineering controls (i.e., factories and equipment) must be observed at all times.

Safety recommendations for biological Laboratories conducting EVD diagnostic tests (with appropriate biosafety BSL3/BSL4 facilities):

Safety recommendations for biological Laboratories conducting EVD diagnostic tests (not equipped with appropriate biosafety BSL3/BSL4 facilities):

Note: All liquids and solid waste should be treated with care and properly disinfected. The specimen container and the laboratory surface should be properly disinfected.

3. Laboratory Diagnostics

Two negative RT-PCR test results, at least 48 hours interval, patients from hospital discharge need a clinical symptom observation.

Ebola virus Lab-broken