Research experience of netizens

Collation
When I started my experiment, people thought I would do what I did. Every day I went to the test bench late at night and thought it was a research task. Two months later, I suddenly found myself standing in the same position. That feeling can only be described as "frustrated. I began to doubt my behaviors and ideas. I feel that the power of habit is binding me. I am used to people and cloud, and I am used to serving and busy. I am not a graduate student, but a cheap and diligent technician. Later, I was surprised to see that my fellow students are repeating the same mistake. So I decided to sum up the experience in scientific research and discuss it with you. It is hoped that this will approach scientific research in the true sense. It is hoped that these experiences will become the accumulation of graduate students after a successful trial, so that later people can learn from them and avoid detours and go straight to the essence of the problem.
Thanks to the clove garden, I can think deeply here.
Is in order.

My experiences in Scientific Research (1)

1. Experiment is performed in half time, and literature is read in half time.
Never spend all the time on the test bench. Reading documents, reading books, reading others' operations, listening to others' experiences, studying others' ideas, and thinking. You must learn comparison, not blindly follow. Otherwise, it will be plagued by some small problems for a long time.
2. The better the preparation, the smoother the experiment.
The ancients cloud, sharpen the knife without mistake cut firewood. Early knowledge reserves, document reserves, material preparation, and method preparation can prevent you from getting confused. Full pre-experiment gives you confidence. One step at a time, you do not have to "start from scratch ". The most intolerable part is the laziness in the first few steps, which may lead to some insurmountable obstacles.
3. The record is authentic and detailed.
People are always a bit vanity. It is the practice of many people to only record successful steps or beautiful results to the experiment records. As you may not know, many valuable experiences and unexpected discoveries have passed. An objective, authentic, and detailed record is a valuable asset.
4. Do not save money for the boss.
Efficiency first. It's time-consuming to save money all day. Once an unreliable item is used, it will only waste time and suffer a blow, and it will not save a penny in the end.
5. Grasp the psychological advantages.
All people who have done experiments have experienced failures and setbacks. Some failures should occur in the pre-experiment phase. You can accept them with confidence. If you do not do a pre-experiment, your frustration will be obvious in the formal experiment. If you misoperate because you are in a hurry, you will be frustrated. If you are able to put it bluntly because you are currently frustrated, you can avoid the occurrence of tragedy. If you do not know what to do before entering the lab in the morning, you 'd better think about it again. The biggest mistake is repeating the same mistake. Remember, it is not suitable for lab practitioners.
Liangice
You are right. I think that although my project is not perfect, I have learned a lot. I agree with what you said. I also want to add two more points. In order to meet the scientific research conditions and seek help everywhere, I have to improve my introverted personality. In addition, I feel that I have become very strong. Do you feel the same way?
Csust
Query and view documents 70%, experiment 30%
Dqyxie
I found some problems in the clinic and read the literature. Although the papers in this field have been published in foreign countries, they were not published at that time (one year ago ). I have not read many documents before I confirm the question. I read the document to find out if someone has done it. My experience is that scientific research should be closely integrated with clinical practice. I am a clinical doctor, so I feel this way.
Huitu_2000
Like doing scientific research, we also need to have a plan and don't have to worry about it. However, we always have to be steadfast in learning. Now I am in the clinical practice of acupuncture, and I want to start from the basics of setting up a needle, this is probably the case in the world
Taotao
I 'd like to add some of my experiences:
When your boss is not clear about the details of your experiment or your boss is not really running in your experiment field, please note: when the experiment is not satisfactory, when there are no expected results, do not repeat the experiment too much. In particular, the boss said to you, "repeat the experiment and do more! "At this time, the first step is to first repeat the experiment details, especially the details or key steps that have not been noticed in the literature, and the second is to prove the experiment results using different methods, sometimes the expected results are covered by a large number of background information, and other methods may be expressed in other ways. Third, you must dare to deny the boss's judgment, of course, this is very difficult in action, but you can prove yourself when the boss is not there.

My experiences in scientific research (2)

1. Let's take a look at the summary first, and then look at the discussion.
View the summary to find out the concept and view the methods
2. First read the previous articles published by the mentor, and then read the papers answered by the Senior brother and sister.
The former knows the general direction (in fact, they should be viewed before their graduate students), and the latter knows which ones can be used for reference.
3. Early Start
Learn key technologies before leaving
4. If you want to continue with your work, you must check the lab records.
Previous results are not necessarily credible!
5. Prepare both hands
To clarify the problem, design subjects can write articles whether positive or negative. What is positive and what is negative.
If the subject requires a positive result, you may need to design a few parts in advance. In case the first part cannot get the expected result, you can use other parts to compensate for the loss.
Litiechen
You are welcome to send more such posts in the future, hoping to see your experience series (3), (4)... as soon as possible )....... Please read the replies in your post frequently and answer questions from your comrades in arms.
Dwliang
Thank you very much for your kindness and love.
My experience is concentrated in scientific research practice for several years.
Some are summarized from your own experiences, and some are summarized from your experiences.
Click it. For example, "Early Start" implies that you may have full preparation, but you may also have the opportunity to fail again, and you may be able to work for a job or contact the country before graduation.
The purpose of my writing is to make more people mature one day earlier and avoid detours.
I did not ignore my questions.
Their opinions prompted me to continue thinking and include more practical and understandable experiences in future (3) (4.
Welcome to the discussion and make progress together.

My experiences in Scientific Research (3)

1. Read the abstract in most articles and the full text in a few articles
After mastering the full-text Query Technique, I often enjoy the full text, so that I don't have time to read the content of the article, so I can't help but read the abstract. There are not many useful full texts. It is a waste to pursue the full text. Of course, reading the abstract is not correct.
2. concentrated time to view documents
Always forget. The more time the document is viewed, the more time the document is wasted. Concentrated time is more easily linked to form an overall impression.
3. Record and Mark
Documents copied or printed are directly marked or annotated with a pen. Document in PDF or HTML format can be highlighted or changed in the text color in the editor. This is another important way to avoid wasting time. Otherwise, you will not see it.
4. Read the articles to be referenced.
The conversion causes are too many examples.
5. Pay attention to the reference value of the article.
The influence factors of publications and the number of cited articles can reflect the reference value of the articles. However, pay attention to how other articles cited in this article evaluate this article: Support, opposition, supplement, and correction.
Pwj918
How can I change the color or highlight of PDF text.
Midas
We have snatched pwj918 microphones:
You must install Adobe batch bat5.0 instead of Acrobat Reader.
The toolbar of Adobe batch bat5.0 contains an icon like a marker!

My experiences in Scientific Research (4)

1. Lab courses cannot be used
Before the lab class, the teacher completed the previous steps and solved all the problems. During the class, we were able to see the perfect last step of the performance. Is this a real experiment? At that time, we really asked the teacher: can we leave? When should I submit the lab report? For most people, lab skills can only be "bubble" in the lab.
2. Communication is the best teacher.
Lab difficulties are common. What is your first response? Retry? Give up? Reading books? These practices all make sense, but the first thing we should think of is communication. Private consultations with people with identities reflect your respect for them. Public discussions with people of the same seniority allow everyone to speak freely and exercise caution. Never build a car behind closed doors. An experiment has been around for half a year. When someone tells you that it is a dead end, isn't it?
3. The highest level of competence is expressive ability.
The best job is ultimately approved by others. The expressive ability is embodied in the writing and speaking ability, and is a quality that needs to be cultivated for a long time. For example, if you find a rare case, you can write an article. If you cannot write it, you can only report one case. For example, if you have prepared a topic and published one or more articles, you can only write a summary or be decompressed. A graph and a table are not expressions. Bidding documents with hundreds of thousands of words can win a large fund. Although the relationship is very important, it cannot be written too poorly. Some people say that I don't want to learn PCR, I don't want to learn SPSs. I just need to learn PPT. It makes sense that the bosses in the lab are walking on the surface by a string of PPT slides. It is common for graduate students to be awe-inspiring because the agile thinking regulations are clear. It is also often hard for graduate students to understand. "Why did my boss write an article when I did most of my work? Let him speak at the conference? "
Didn't you see anyone else have the ability to speak?
4. learn English well.
Now, academic exchange is already in the English world, both in Japan and Europe. You don't have to be sorry for not reading a French article. The person who writes the article is worried about not learning English well. If you are not proficient in English, do not study for the moment.
5. Sci is a trap.
Luck! Someone did not bother to publish an Sci article. You should not envy him. As far as I know, the subject was designed by others, and the experiment was hand-picked by others. The article was rewritten by a mentor who helped him find someone outside China, and he was not good at scientific research. However, because of an Sci article, he thought he could show his skills in the scientific research world. So I want to go abroad, do experiments, continue to issue SCI, and earn dollars. From then on, he embarked on a path that does not belong to him. (It may be caused by sour grape psychology ). But looking at people around you and looking at yourself, is it really suitable for scientific research? Is it really not suitable for scientific research?
Litiechen:
Dwliang said well.
Lab skills can only be "bubble" in the lab.
The word "bubble" tells the essence, and many people are eager to achieve it quickly, but the experiment skills are indeed "bubble. Professor Sheng zujia of Fudan University spent 15 years cultivating E. coli and familiarized itself with the properties of E. coli, laying a solid foundation for future experiments, this skill and patience may be the best teacher for few people to communicate with each other. Today, confidentiality is prevalent, and many people do not want to know what to do. I can't figure it out all the time. Do you have an idea to help you improve it? If you cannot implement it, isn't it good for others to turn your thoughts into reality? The most important thing is that you can also learn how to turn your ideas into reality.
The highest level of ability is the ability to express
I have different opinions on this. expressive ability is indeed important, but it is not the only one. experimental data is the most important. A person should still do some work in a down-to-earth Manner. Superficial articles can only be done for the moment, not for the whole world. Never be jealous of people who make superficial articles; otherwise, it will be too late when you regret it. Actually, you will get what you deserve.
Dwliang
About: the highest level of competence is expressive ability.
I wrote this sentence to emphasize the importance of expression ability.
In fact, it is the most important thing to do things in a down-to-earth manner. The opposite is impetuous.
The relationship between data and expression is like the relationship between economic foundation and superstructure.
Virus
I cann't agree it more !!!!!
Uhouuhou
I admire the author but have a little bit
1. Lab courses cannot be used
Before the lab class, the teacher completed the previous steps and solved all the problems. During the class, we were able to see the perfect last step of the performance. Is this a real experiment? At that time, we really asked the teacher: can we leave? When should I submit the lab report?
For most people, lab skills can only be "bubble" in the lab. "
I think the lab course is very important. After all, I participated and practiced it myself. In practice, there must be a problem. Since you have no problems, you may not be able to see others, and other people's problems may be your future problems. I think at least we must carefully preview the course before class. during class, we need to "install, sell, and sell silly" so that the teacher can say more times where you want to stop. Don't go after the lecture. Let's take a look at what others have not done, especially what they have done, and what they have done to help them solve the problem. At the same time, they have also improved themselves.

My experiences in Scientific Research (5)

I don't know to what extent scientific research can tolerate fraud, and whether scientific research and counterfeiting are twins.
But all the people on the platform say-never fake it.
1. Proper counterfeiting: it is irrelevant and the deviation is not large.
In this paper, we compared normal adult DNA with age and gender matching, and actually compared it with cord blood DNA. In this paper, we performed 200 normal cases and 150 actual cases; there are significant differences in the paper, but there are also significant differences in reality, but the difference in mean is not as big as in the paper; In the paper, the random grouping, in fact, random grouping; the paper to the animals for Aseptic Operation, actually, only some devices are soaked in disinfectant.
2. Passive counterfeiting.
Brother Yi's paper was published, and his mentor asked Yi to continue. He did not repeat it, but he could not say that he had a problem with his paper, yi has done more "in-depth" Research on the basis of concealing the facts; the mentor wants the results, and Iraq can make the results; many people's experiments have become exceptionally smooth several months before graduation, all the items that come out are coming out.
3. Objective fraud: no intention, no knowledge.
In this paper, a new missing mutation is reported. It is said that only one clone sequencing is selected, and an experiment that should have been repeated several times has not been repeated.
4. Active fraud: quick success, no rain or shine.
One of the electrophoresis photos in this paper is too late to be redone and replaced by a similar photo from others. When the enzyme is cut, one of the bands should be completely cut off, but it is always not clean, I used Photoshop to paint it out. The PCR workload in this paper is large, but the registration book of pcrmeter is only one record. In this paper, P = 0.041, the actual P = 0.055, remove a positive sample from the control group and get 0.041.
5. Positive counterfeiting: Chasing fame and fortune, almost crazy. Yi published 50 papers with the first signature in a year. There were first papers with experimental records.
Cider
Don't be so honest. What are you doing to tell the truth. However, to be honest, I don't feel very interesting. How many points do you give it to me when you look up the document? 5 points? It is a waste of my life.
Sunnyfff
I only know that most of the documents in China are untrusted. It is normal for me to refer to the documents and make it clear that you have problems.

My experiences in Scientific Research (6)

English article writing
1. read 10 documents and summarize 100 commonly used sentence patterns and phrases. Review Frequently.
Note that the author of the document must be an English speaker and the document content must be related to your major.
This is a by-product of literature reading at ordinary times.
2. Find 3-5 articles about the technical route and statistical methods that are close to your subject and read them carefully.
Write a draft of the paper. The subject, author, abstract, background, purpose, materials, methods, results, discussions,
A unified format of acknowledgment, reference, legend, graph, table, photo, and description. The advantage of doing so is that it can
In order to easily change to any magazine format.
Http://www.jiansuo.net/cgi-bin/ut/topic_show.cgi? Id = 10853 & H = 1 & BPG = 1 & age = 0
3. Find 5-8 documents for each part of the thesis, especially some specific methods and specific aspects to be discussed.
Read, enrich and improve.
Only English expressions are involved in the discussion, and only English writing experience is recommended.
4. Find the draft of the magazine you want to submit, find 2-3 articles about the magazine, and rewrite the article according to its format.
Note: Each rewrite must be saved as a different file name, so that the file cannot be recovered.
5. Find an English expert. If you cannot find a suitable person, go to http://www.oleng.com.au/. the company's English document editing service (English correction and improvement, not translation), which is strongly recommended to those who have no time. It took only two months for my colleagues to contribute to the magazine and publish the magazine. I spent $200 in the middle and changed the English in this company. It was very fast.
Dwliang
The website seems to have changed a lot. I have not studied the details carefully. You can only read it carefully or ask by email.
My experiences in Scientific Research (7)
Document management
1. When downloading the electronic document (CAJ, PDF, HTML), paste the article question as the file name. In this way, you can see it clearly.
Note: The file name cannot have special characters \/:*? <> | And linefeed deleted.
2. Store different topics in different folders. The folder question should be brief, such as PD, LTP, PKC, and no.
3. The documents you have read should be classified into different subfolders, at least to separate useful and useless.
4. Add the 001,002,003 number before the file name based on the importance of important documents, and arrange the icons by name. The most important documents are listed at the beginning.
5. Copy or print the documents, and punch them into a hard folder (￥10-20/RMB 10-20) with a punching tool (￥10-15 ). To avoid loss.
My experiences in Scientific Research (8)
We often make some clever use of references, but these will reduce the quality of the paper.
1. Do not reference
I learned from similar jobs in my peers, but deliberately did not reference similar jobs in my peers, making my work seem "novel" and "leading ". In fact, the reviewers are peer reviewers.
2. Get out of context
Deliberately intercept the parts that the author tries to deny to outshine his opinion.
3. Incorrect Guidance
The original document is not carefully read, and the citation is incorrect.
4. Incorrect Source
Some words are from unreliable sources (such as informal or non-academic publications) and do not indicate sources. Common statistics.
5. Blind self-Citation
It is not to describe the relationship between your own work and your previous work, but to increase the number of times your articles are cited.
Yinian
In fact, not necessarily peer reviewers
Litiechen
Yinian said: In fact, not only do the reviewers not necessarily come from experts in this field, but the review experts from scientific research are mostly from outside the field. Therefore, I am writing an opening report, when time permits, I often ask for advice from senior medical undergraduates to see if they think my question is "advanced and not advanced ". This operation may have unexpected results. This is my experience. Interested comrades may wish to give it a try.
.

My experiences in Scientific Research (9)

Reasons for the low level of domestic articles:
1. Outdated reviewer knowledge
The ability of older reviewers to query documents and access the Internet is quite limited. It is impossible to check whether the research is meaningful, where the innovation points are, whether the method is reliable, and whether the results are credible. However, what they often comment on is that "references are not new ".
2. Select the wrong reviewer
Although two reviewers are generally designated, the editorial department often asks people who do not understand molecular biology to review the articles of molecular biology, so that those who do not know statistics can review the articles that are complicated to process. Out of love for face, few people propose that "I am not suitable for reviewing this Article ".
3. Link articles
Everything is simple.
4. Do not acknowledge negative results
Honest negative results are considered meaningless. No wonder someone shouted, "I want to run a negative magazine ".
5. Fraud
No one is willing to become a victim of the system. The expected results of the Failover cannot be outputted. To survive, to graduate on schedule.
Litiechen
References are not new enough
Dwliang wrote well and hoped to continue his "experience". At the same time, he hoped that all comrades-in-arms would take a closer look and talk about their views based on their own experiences, so that their comrades-in-arms could improve together.

My experiences in Scientific Research (10)

Dynamic Research
1. Scientific research depends on accumulation.
As lunqin discovered, there were fewer and fewer opportunities to win the Nobel Prize with a simple observation like X-ray. More scientific research results come from long-term laboratory accumulation. Ultimately, it is named. Do not count on one day for scientific research. Design issues should not be too ambitious. The same is true for fund reviews. There is no accumulation in the early stage, and the possibility of obtaining funding is small. Think about choosing a Mentor: Do you want to start from scratch or marry yourself?
2. Documents should be tracked.
When you start a question, you can check the document to understand the situation, and the conclusion may be very different. Follow up during the experiment. If you are lucky, you can get more clues. Correct Your experiment accordingly. You must review the document before writing the paper.
3. Review the record.
The previous lab records should be reviewed frequently. As your experience increases and your understanding increases, you may find that your initial judgment may not be correct.
4. material deterioration.
Over time, some reagents will degrade, and some devices will age, which leads you to fail to get the previous results after fully following the previous procedure. PCR is a devil! Many people feel this way. This is just a simple example. If a reagent only reduces the effective amount, you need to increase the dosage. If harmful substances are generated after deterioration, I am afraid it is time to change the reagent. If the device reading is drifting, you must correct it. In short, if a problem occurs, find the cause. "exact" is required for each step "! Otherwise, it is a sword.
5. The boss needs to see it clearly.
The boss also crossed the river by feeling the stones. His thoughts are changing at any time. If something is not good, he will turn. You need to polish your eyes to see the symptoms, or else it will become dizzy. It can be properly guided. Interaction.
Keep Pace with the Times! Do a good job in scientific research!